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mouse fcγriv adcc reporter bioassay kit  (Promega)

 
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    Structured Review

    Promega mouse fcγriv adcc reporter bioassay kit
    (A) Five humanized CV804 antibodies were analyzed by flow cytometric assay of titrated candidates against SARS-CoV-2 spike protein expressing cells. Four parameter logistic curve fitted with EC 50 in parentheses. Normalized by secondary alone and saturation signal. Representative data of two independent experiments are shown. (B) Evaluation of hCV804-40 antibody, REGN10987, and LY-CoV1404 antibody binding to cells infected with SARS-CoV-2 variants. ADCC activity against spike-expressing cells for mouse derived antibodies. (D) In vitro cell infection inhibition experiments were conducted. The viral strains used were hCoV-19/Japan/TY/WK-521/2020 (top) and SARS-CoV-2/Japan/TY-501/2020 (bottom), with VeroE6/TMPRSS2 cells being used as the target cells. The hCV804-35 antibody, REGN10987, and LY-CoV 1404 were measured in this experiment. The antibody concentration that inhibited cell death by 50% was defined as the IC 50 . " width="250" height="auto" />
    Mouse Fcγriv Adcc Reporter Bioassay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+fc%CE%B3riv+adcc+reporter+bioassay+kit/mouse+fc%CE%B3riv+adcc+bioassay/pmc11611099-266-5-11
    Average 90 stars, based on 1 article reviews
    mouse fcγriv adcc reporter bioassay kit - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Discovery of anti-SARS-CoV-2 S2 protein antibody CV804 with broad-spectrum reactivity with various beta coronaviruses and analysis of its pharmacological properties in vitro and in vivo"

    Article Title: Discovery of anti-SARS-CoV-2 S2 protein antibody CV804 with broad-spectrum reactivity with various beta coronaviruses and analysis of its pharmacological properties in vitro and in vivo

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0300297

    (A) Five humanized CV804 antibodies were analyzed by flow cytometric assay of titrated candidates against SARS-CoV-2 spike protein expressing cells. Four parameter logistic curve fitted with EC 50 in parentheses. Normalized by secondary alone and saturation signal. Representative data of two independent experiments are shown. (B) Evaluation of hCV804-40 antibody, REGN10987, and LY-CoV1404 antibody binding to cells infected with SARS-CoV-2 variants.
    Figure Legend Snippet: (A) Five humanized CV804 antibodies were analyzed by flow cytometric assay of titrated candidates against SARS-CoV-2 spike protein expressing cells. Four parameter logistic curve fitted with EC 50 in parentheses. Normalized by secondary alone and saturation signal. Representative data of two independent experiments are shown. (B) Evaluation of hCV804-40 antibody, REGN10987, and LY-CoV1404 antibody binding to cells infected with SARS-CoV-2 variants. "+++", "++", "+" indicate positive binding; "-" indicates negative binding. (C) Evaluation of antibody-dependent cell-mediated cytotoxicity ADCC activity against spike-expressing cells for mouse derived antibodies. (D) In vitro cell infection inhibition experiments were conducted. The viral strains used were hCoV-19/Japan/TY/WK-521/2020 (top) and SARS-CoV-2/Japan/TY-501/2020 (bottom), with VeroE6/TMPRSS2 cells being used as the target cells. The hCV804-35 antibody, REGN10987, and LY-CoV 1404 were measured in this experiment. The antibody concentration that inhibited cell death by 50% was defined as the IC 50 .

    Techniques Used: Flow Cytometry, Expressing, Binding Assay, Infection, Activity Assay, Derivative Assay, In Vitro, Inhibition, Concentration Assay



    Similar Products

    mouse fcγriv adcc reporter bioassay kit  (Promega)
    90
    Promega mouse fcγriv adcc reporter bioassay kit
    (A) Five humanized CV804 antibodies were analyzed by flow cytometric assay of titrated candidates against SARS-CoV-2 spike protein expressing cells. Four parameter logistic curve fitted with EC 50 in parentheses. Normalized by secondary alone and saturation signal. Representative data of two independent experiments are shown. (B) Evaluation of hCV804-40 antibody, REGN10987, and LY-CoV1404 antibody binding to cells infected with SARS-CoV-2 variants. ADCC activity against spike-expressing cells for mouse derived antibodies. (D) In vitro cell infection inhibition experiments were conducted. The viral strains used were hCoV-19/Japan/TY/WK-521/2020 (top) and SARS-CoV-2/Japan/TY-501/2020 (bottom), with VeroE6/TMPRSS2 cells being used as the target cells. The hCV804-35 antibody, REGN10987, and LY-CoV 1404 were measured in this experiment. The antibody concentration that inhibited cell death by 50% was defined as the IC 50 . " width="250" height="auto" />
    Mouse Fcγriv Adcc Reporter Bioassay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+fc%CE%B3riv+adcc+reporter+bioassay+kit/mouse+fc%CE%B3riv+adcc+bioassay/pmc11611099-266-5-11
    Average 90 stars, based on 1 article reviews
    mouse fcγriv adcc reporter bioassay kit - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Five humanized CV804 antibodies were analyzed by flow cytometric assay of titrated candidates against SARS-CoV-2 spike protein expressing cells. Four parameter logistic curve fitted with EC 50 in parentheses. Normalized by secondary alone and saturation signal. Representative data of two independent experiments are shown. (B) Evaluation of hCV804-40 antibody, REGN10987, and LY-CoV1404 antibody binding to cells infected with SARS-CoV-2 variants.

    Journal: PLOS ONE

    Article Title: Discovery of anti-SARS-CoV-2 S2 protein antibody CV804 with broad-spectrum reactivity with various beta coronaviruses and analysis of its pharmacological properties in vitro and in vivo

    doi: 10.1371/journal.pone.0300297

    Figure Lengend Snippet: (A) Five humanized CV804 antibodies were analyzed by flow cytometric assay of titrated candidates against SARS-CoV-2 spike protein expressing cells. Four parameter logistic curve fitted with EC 50 in parentheses. Normalized by secondary alone and saturation signal. Representative data of two independent experiments are shown. (B) Evaluation of hCV804-40 antibody, REGN10987, and LY-CoV1404 antibody binding to cells infected with SARS-CoV-2 variants. "+++", "++", "+" indicate positive binding; "-" indicates negative binding. (C) Evaluation of antibody-dependent cell-mediated cytotoxicity ADCC activity against spike-expressing cells for mouse derived antibodies. (D) In vitro cell infection inhibition experiments were conducted. The viral strains used were hCoV-19/Japan/TY/WK-521/2020 (top) and SARS-CoV-2/Japan/TY-501/2020 (bottom), with VeroE6/TMPRSS2 cells being used as the target cells. The hCV804-35 antibody, REGN10987, and LY-CoV 1404 were measured in this experiment. The antibody concentration that inhibited cell death by 50% was defined as the IC 50 .

    Article Snippet: ADCC activity was measured by mouse FcγRIV ADCC Reporter Bioassay kit (Promega) according to manufacturer’s instruction.

    Techniques: Flow Cytometry, Expressing, Binding Assay, Infection, Activity Assay, Derivative Assay, In Vitro, Inhibition, Concentration Assay